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The easiest way to tell is to run a file with and without the object and check the zone/system sizing details in the eio file. I ran the example file 5ZoneNightVent2.idf and then again without the ZoneCrossMixing objects and do see a very small difference in zone loads and zone design air flow. Even though the ZoneCrossMixing objects mixed between Space2-1 and Space4-1 this change had a small impact on all zones (less than 1 Watt or 0.03%). Red is the original file and yellow is the file without the ZoneCrossMixing objects.

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System sizing will also be affected when zone design air flow rates change.

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